Tissue localization and the establishment of a sensitive immunoassay of the newly discovered human phospholipase B-precursor (PLB-P).

Shengyuan Xu, Linjun Cai, Linshu Zhao, Lena Douhan-Håkansson, Gudjon Kristjánsson, Karlis Pauksen, Per Venge

Dept. of Medical Sciences, Clinical Chemistry, Uppsala University, SE-751 85, Uppsala, Sweden. shengyuan.xu@medsci.uu.se

Journal of immunological methods 2010 Feb 28

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Human phospholipase B-precursor (PLB-P) is a newly identified and purified protein from human neutrophils. The precise function of PLB-P in vivo is not yet known. Its existence in neutrophils and the enzymatic activity against phospholipids imply a role in the defence against invading microorganisms and in the generation of lipid mediators of inflammation. We describe here the generation of specific antibodies against PLB-P, the tissue localizations of PLB-P and the establishment of an accurate, specific, and reproducible radioimmunoassay (RIA). A survey of normal and malignant tissues showed strong immunostaining of PLB-P in neuronal and myeloid cells and in adrenal glands. Elevated levels were found in sera of patients with influenza A infection i.e. >1 microg/L and in gut fluids of patients with inflammatory bowel disease i.e. >20 microg/L. The levels correlated to markers of neutrophil activation, suggesting a neutrophil origin of PLB-P in these conditions. The antibodies and the assay will be useful in the future basic and clinical investigations of PLB-P. 2010 Elsevier B.V. All rights reserved.

Citation

Shengyuan Xu, Linjun Cai, Linshu Zhao, Lena Douhan-Håkansson, Gudjon Kristjánsson, Karlis Pauksen, Per Venge. Tissue localization and the establishment of a sensitive immunoassay of the newly discovered human phospholipase B-precursor (PLB-P). Journal of immunological methods. 2010 Feb 28;353(1-2):71-7