Crystallization and preliminary X-ray analysis of Psu, an inhibitor of the bacterial transcription terminator Rho.

Susmita Khamrui, Amitabh Ranjan, Bibhusita Pani, Ranjan Sen, Udayaditya Sen

Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhan Nagar, Kolkata 700 064, India.

Acta crystallographica. Section F, Structural biology and crystallization communications 2010 Feb 1

filter terms:

Psu, a coat protein from bact eriophage P4</a>, inhibits Rho-dependent transcription termination both in vivo and in vitro. The Psu protein is alpha-helical in nature and appeared to be a dimer in solution. It interacts with Rho and affects the ATP binding and RNA-dependent ATPase activity of Rho, which in turn reduces the rate of RNA release from the elongation complex. Crystals of Psu were grown in space group I422 in the presence of PEG, with unit-cell parameters a = b = 148.76, c = 63.38 A and a calculated Matthews coefficient of 2.1 A(3) Da(-1) (41.5% solvent content), assuming the presence of two molecules in the asymmetric unit. A native data set was collected to 2.3 A resolution.

Citation

Susmita Khamrui, Amitabh Ranjan, Bibhusita Pani, Ranjan Sen, Udayaditya Sen. Crystallization and preliminary X-ray analysis of Psu, an inhibitor of the bacterial transcription terminator Rho. Acta crystallographica. Section F, Structural biology and crystallization communications. 2010 Feb 1;66(Pt 2):204-6