J M Talia, M A Alvarez, N B Debattista, N B Pappano
Laboratory of Physical Chemistry, Department of Chemistry, San Luis National University, San Luis, Argentina.
Folia microbiologica 2009 NovIn order to determine the existence of synergism of the bacteriostatic action of flavonoids against G(+) bacteria between a clinically interesting conventional antibiotic and a flavonoid, combinations of oxacillin (OXC) and 2,4-dihydroxychalcone (DCH) as enhancer were assayed against methicillin-sensitive Staphylococcus aureus ATCC 29 213 and methicillin-resistant S. aureus ATCC 43 300. Using a kinetic-turbidimetric method, growth kinetics was monitored in a broth containing variable amounts of OXC alone and combinations of variable OXC-constant DCH. The minimum inhibitory concentrations (MIC) of OXC alone and in combination with DCH were evaluated. For the 29 213 strain, OXC MIC was 25 microg/mL, while combinations of 2-8 microg/mL OXC with 10 microg/mL of DCH totally inhibited growth and showed synergism. The resistance of the 43 300 strain in the presence of OXC was verified; OXC-DCH combinations decreased bacterial growth by 35 %. DCH augments the action of OXC against methicillin-susceptible S. aureus and therefore constitutes a good bacteriostatic agent for methicillin-resistant S. aureus.
J M Talia, M A Alvarez, N B Debattista, N B Pappano. Susceptibility of Staphylococcus aureus strains toward combinations of oxacillin-2,4-dihydroxychalcone. Folia microbiologica. 2009 Nov;54(6):516-20
PMID: 20140719
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