The early noncoding region of human papillomavirus type 16 is regulated by cytoplasmic polyadenylation factors.

Jacob A Glahder, Karen Kristiansen, Marjorie Durand, Jeppe Vinther, Bodil Norrild

Department of Cellular and Molecular Medicine, Panum Institute, University of Copenhagen, DK-2200 Copenhagen N, Denmark. jglahder@hotmail.com

Virus research 2010 May

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All human papillomavirus type 16 (HPV-16) early mRNAs are polyadenylated at the poly(A) signal within the early 3' untranslated region (3'UTR). The 3'end of the early E5 open reading frame and the 3'UTR of HPV-16 is very AU-rich, with five regions similar to cytoplasmic polyadenylation elements (CPEs). We show here that a fragment of the early 3'end comprising four of the five CPE-like regions when inserted downstream of a reporter gene confers regulation of the gene expression. A key protein involved in cytoplasmic polyadenylation is CPEB. We show that the human CPEB1 can repress the activity of the reporter construct containing the HPV-16 early sequences. This repression can be counteracted by a human cytoplasmic poly(A) polymerase, hGLD-2 fused to CPEB1. The hGLD-2/CPEB1 fusion protein facilitates furthermore poly(A) elongation of early HPV transcripts. (c) 2010 Elsevier B.V. All rights reserved.

Citation

Jacob A Glahder, Karen Kristiansen, Marjorie Durand, Jeppe Vinther, Bodil Norrild. The early noncoding region of human papillomavirus type 16 is regulated by cytoplasmic polyadenylation factors. Virus research. 2010 May;149(2):217-23