Purification and characterization of a beta-glucosidase from the root parasitic plant Orobanche minor Sm.

Izumi Sasanuma, Go Hirakawa

Oyama National College of Technology, Oyama, Japan. sasaki@oyama-ct.ac.jp

Bioscience, biotechnology, and biochemistry 2010

filter terms:

The beta-glucosidase of a root parasitic angiosperm, Orobanche minor Sm., was purified and characterized. The optimum pH and temperature for activity of the enzyme were 5.0 and 50 degrees C. The beta-glucosidase was stable at up to 50 degrees C at pH 4.0-10.0. The M(r) was estimated to be 33 kD by SDS-PAGE. The enzyme hydrolyzed p-nitrophenyl-beta-D-glucopyranoside and salicin, but not the cell wall of O. minor or cellohexaose.

Citation

Izumi Sasanuma, Go Hirakawa. Purification and characterization of a beta-glucosidase from the root parasitic plant Orobanche minor Sm. Bioscience, biotechnology, and biochemistry. 2010;74(3):646-8