PDGF and bFGF modulate tube formation in adipose tissue-derived stem cells.

Silke Keerl, Sebastian Gehmert, Sanga Gehmert, Yao-Hua Song, Eckhard Alt

Department of Molecular Pathology, University of Texas MD Anderson Cancer Center, Houston, TX 77054, USA.

Annals of plastic surgery 2010 Apr

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Recent evidence indicates that mesenchymal stem cells contribute to tumor angiogenesis through yet undefined mechanisms. In the present study, we investigated the angiogenic properties of human adipose tissue-derived stem cells and the mechanisms involved. Freshly isolated human adipose tissue-derived stem cells were seeded onto wells coated with Matrigel and cultured in endothelial growth medium. Capillary-like tube formation was observed after 18 hours culture. Tube formation was significantly reduced in the presence of antibodies against platelet-derived growth factor receptor beta or basic fibroblast growth factor. Collectively, these data suggest that freshly isolated adipose tissue-derived stem cells have the capacity to differentiate into capillary structures and platelet-derived growth factor and bFGF plays a critical role in this process.

Citation

Silke Keerl, Sebastian Gehmert, Sanga Gehmert, Yao-Hua Song, Eckhard Alt. PDGF and bFGF modulate tube formation in adipose tissue-derived stem cells. Annals of plastic surgery. 2010 Apr;64(4):487-90