Assays of the helicase, ATPase, and exoribonuclease activities of the yeast mitochondrial degradosome.

Michal Malecki, Piotr P Stepien, Pawel Golik

Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Pawinskiego 5A, 02-106, Warsaw, Poland.

Methods in molecular biology (Clifton, N.J.) 2010

filter terms:

The mitochondrial degradosome (mtEXO) is the main enzymatic complex in RNA degradation, processing, and surveillance in Saccharomyces cerevisiae mitochondria. It consists of two nuclear-encoded subunits: the ATP-dependent RNA helicase Suv3p and the 3' to 5' exoribonuclease Dss1p. The two subunits depend on each other for their activity; the complex can therefore be considered as a model system for the cooperation of RNA helicases and exoribonucleases in RNA degradation. All the three activities of the complex (helicase, ATPase, and exoribonuclease) can be studied in vitro using recombinant proteins and protocols presented in this chapter.

Citation

Michal Malecki, Piotr P Stepien, Pawel Golik. Assays of the helicase, ATPase, and exoribonuclease activities of the yeast mitochondrial degradosome. Methods in molecular biology (Clifton, N.J.). 2010;587:339-58