The novel association of the chemokine CCL22 with abdominal aortic aneurysm.

Jonathan Golledge, Paula Clancy, Corey Moran, Erik Biros, Catherine Rush, Philip Walker, Paul Norman

Vascular Biology Unit, Department of Surgery, School of Medicine, James Cook University, Townsville, QLD, Australia. jonathan.golledge@jcu.edu.au

The American journal of pathology 2010 May

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The aim of this study was to examine aortic biopsies with a cytokine array to identify new cytokines associated with abdominal aortic aneurysm (AAA). We assessed the relative expression of 79 cytokines using antibody-based cytokine arrays in a total of 12 AAA and 12 control aortic biopsies. Based on these findings we validated the findings for one cytokine by examining a further 11 AAA and 11 atherothrombosis biopsies and serum from 1028 men, 315 of whom had an AAA. Three cytokines (interleukins 1B and 8, and Chemokine CC motif ligand 22 [CCL22]) were consistently up-regulated in AAA biopsies. Since CCL22 had not previously been associated with aortic dilatation, we confirmed the upregulation of this cytokine in further tissue biopsies and serum using enzyme-linked immunosorbent assay. Median serum concentrations of CCL22 were greater in men with AAA (0.69 ng/ml) than controls (0.56 ng/ml, P < 0.01). Serum CCL22 was independently associated with both small (OR 1.51, 95% CI 1.21-1.88) and large AAA (OR 1.33, 95% CI 1.08-1.62) after adjusting for other risk factors. The association between CCL22 and AAA was also confirmed using immunohistochemistry. The results presented in this study demonstrate a novel association between CCL22 and AAA as well as illustrate how a protein array can be used to identify novel markers of potential pathogenic and diagnostic significance for AAA.

Citation

Jonathan Golledge, Paula Clancy, Corey Moran, Erik Biros, Catherine Rush, Philip Walker, Paul Norman. The novel association of the chemokine CCL22 with abdominal aortic aneurysm. The American journal of pathology. 2010 May;176(5):2098-106