Visualisation of lymphoid organ development.

Henrique Veiga-Fernandes, Katie Foster, Amisha Patel, Mark Coles, Dimitris Kioussis

Division of Molecular Immunology, MRC National Institute for Medical Research, Mill Hill, London, UK.

Methods in molecular biology (Clifton, N.J.) 2010

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This chapter provides information on imaging tools that can be employed to visualise and study lymphoid organ development. We focus on the use of genetically modified mouse models that take advantage of fluorescent protein expression in discrete cell populations, thus allowing live cell imaging during lymphoid organogenesis. We discuss approaches that allow characterisation of the cell types involved in the formation of lymphoid organs, including (i) functional assays in explant organ cultures and (ii) high-resolution whole-mount immunostaining methods, which are useful for the characterisation of specific cell populations in the context of the whole developing organ.

Citation

Henrique Veiga-Fernandes, Katie Foster, Amisha Patel, Mark Coles, Dimitris Kioussis. Visualisation of lymphoid organ development. Methods in molecular biology (Clifton, N.J.). 2010;616:161-79