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We use holographic video microscopy to track the three-imensional translational and rotational diffusion of copper oxide nanorods suspended in water. Rayleigh-Sommerfeld back-propagation of a single holographic snapshot yields a volumetric reconstruction of the nanorod's optical scattering pattern, from which we estimate both its dimensions and also its instantaneous position and orientation. Analyzing a video sequence yields measurements of the freely diffusing nanorod's dynamics, from which we estimate the technique's resolution.


Fook Chiong Cheong, David G Grier. Rotational and translational diffusion of copper oxide nanorods measured with holographic video microscopy. Optics express. 2010 Mar 29;18(7):6555-62

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PMID: 20389679

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