Determination of p-aminohippuric acid in rat plasma by liquid chromatography-tandem mass spectrometry.

Hsien-Yuan Fan, Chang-Ching Lin, Li-Heng Pao

School of Pharmacy, National Defense Medical Center, Taipei, Taiwan, ROC.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2010 Jun 1

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A rapid, simple and sensitive method was developed for the determination of para-aminohippuric acid (PAH) in rat plasma using liquid chromatography tandem mass spectrometry (LC-MS-MS). Acetaminophen was used as the internal standard. Chromatographic separation was performed using a Symmetry C18 column and the mobile phase was composed of A: 2 mM ammonium formate and 0.1% formic acid in water and B: 2 mM ammonium formate and 0.1% formic acid in acetonitrile (ACN) (A:B, 30:70, v/v). Detection was performed on a triple-quadrupole tandem mass spectrometer using positive ion mode electrospray ionization (ESI) in the multiple reaction monitoring (MRM) mode. The MS/MS ion transitions monitored were m/z 195.2-->120.2 and 152.1-->110.1 for PAH and acetaminophen, respectively. Good linearity is observed over the concentration range of 0.1-500 microg/ml. The method was proved to be accurate and reliable and was applied to a pharmacokinetic study in rat. Copyright 2010 Elsevier B.V. All rights reserved.

Citation

Hsien-Yuan Fan, Chang-Ching Lin, Li-Heng Pao. Determination of p-aminohippuric acid in rat plasma by liquid chromatography-tandem mass spectrometry. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 2010 Jun 1;878(19):1643-6