Development of a gene transfer system for the mycelia of Flammulina velutipes Fv-1 strain.

Tomoko Maehara, Makoto Yoshida, Yasuhiro Ito, Shizuko Tomita, Koji Takabatake, Hitomi Ichinose, Satoshi Kaneko

Food Biotechnology Division, National Food Research Institute, Tsukuba, Ibaraki, Japan.

Bioscience, biotechnology, and biochemistry 2010

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To develop a gene transformation method for Flammulina velutipes, we constructed a vector with hph gene under control of the trp1 gene promoter. The vector was integrated into protoplast derived from mycelia by the calcium-polyethylene glycol method, as it has not been reported for F. velutipes. Transformation efficiency was much improved when transformation was performed by the restriction enzyme mediated integration method.

Citation

Tomoko Maehara, Makoto Yoshida, Yasuhiro Ito, Shizuko Tomita, Koji Takabatake, Hitomi Ichinose, Satoshi Kaneko. Development of a gene transfer system for the mycelia of Flammulina velutipes Fv-1 strain. Bioscience, biotechnology, and biochemistry. 2010;74(5):1126-8