2',4'-Dihydroxychalcone-induced apoptosis of human gastric cancer MGC-803 cells via down-regulation of survivin mRNA.

Chenghua Lou, Guangming Yang, Hao Cai, Mingchang Zou, Zisheng Xu, Yu Li, Fengming Zhao, Weidong Li, Li Tong, Mingyan Wang, Baochang Cai

Nanjing Haichang Chinese Herbal Medicinal Pieces Co, Ltd, Nanjing 210061, China.

Toxicology in vitro : an international journal published in association with BIBRA 2010 Aug

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2',4'-Dihydroxychalcone (TFC), a main component in Herba Oxytropis, is grouped under flavonoids, which are well known to have antitumor activities in vitro. In this study, the possible antitumor mechanism of TFC in human gastric cancer MGC-803 cells is examined. Hoechst 33258 staining analysis indicates that TFC causes MGC-803 cell shrinkage and apoptotic body formation, typical characteristics of apoptosis. Flow cytometric analysis demonstrates that TFC causes cell cycle arrest in the G2/M phase. Furthermore, TFC significantly increases caspase-3 activity but decreases survivin mRNA expression. Therefore, TFC can induce the apoptosis of MGC-803 cells via down-regulation of survivin mRNA expression. Copyright (c) 2010 Elsevier Ltd. All rights reserved.

Citation

Chenghua Lou, Guangming Yang, Hao Cai, Mingchang Zou, Zisheng Xu, Yu Li, Fengming Zhao, Weidong Li, Li Tong, Mingyan Wang, Baochang Cai. 2',4'-Dihydroxychalcone-induced apoptosis of human gastric cancer MGC-803 cells via down-regulation of survivin mRNA. Toxicology in vitro : an international journal published in association with BIBRA. 2010 Aug;24(5):1333-7