Quantitation of the ribosomal protein autoregulatory network using mass spectrometry.

Michael T Sykes, Edit Sperling, Stephen S Chen, James R Williamson

Department of Molecular Biology, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA.

Analytical chemistry 2010 Jun 15

filter terms:

Relative levels of ribosomal proteins were quantified in crude cell lysates using mass spectrometry. A method for quantifying cellular protein levels using macromolecular standards is presented that does not require complex sample separation, identification of high-responding peptides, affinity purification, or postgrowth modifications. Perturbations in ribosomal protein levels by overexpression of individual proteins correlate to known autoregulatory mechanisms and extend the network of ribosomal protein regulation.

Citation

Michael T Sykes, Edit Sperling, Stephen S Chen, James R Williamson. Quantitation of the ribosomal protein autoregulatory network using mass spectrometry. Analytical chemistry. 2010 Jun 15;82(12):5038-45