Transcriptome analysis by cDNA-AFLP of Suillus luteus Cd-tolerant and Cd-sensitive isolates.

Joske Ruytinx, Adrian R Craciun, Karen Verstraelen, Jaco Vangronsveld, Jan V Colpaert, Nathalie Verbruggen

Centre for Environmental Sciences, Environmental Biology Group, Universiteit Hasselt, 3590 Diepenbeek, Belgium.

Mycorrhiza 2011 Apr

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The ectomycorrhizal basidiomycete Suillus luteus (L.:Fr.), a typical pioneer species which associates with young pine trees colonizing disturbed sites, is a common root symbiont found at heavy metal contaminated sites. Three Cd-sensitive and three Cd-tolerant isolates of S. luteus, isolated respectively from non-polluted and a heavy metal-polluted site in Limburg (Belgium), were used for a transcriptomic analysis. We identified differentially expressed genes by cDNA-AFLP analysis. The possible roles of some of the encoded proteins in heavy metal (Cd) accumulation and tolerance are discussed. Despite the high conservation of coding sequences in S. luteus, a large intraspecific variation in the transcript profiles was observed. This variation was as large in Cd-tolerant as in sensitive isolates and may help this pioneer species to adapt to novel environments.

Citation

Joske Ruytinx, Adrian R Craciun, Karen Verstraelen, Jaco Vangronsveld, Jan V Colpaert, Nathalie Verbruggen. Transcriptome analysis by cDNA-AFLP of Suillus luteus Cd-tolerant and Cd-sensitive isolates. Mycorrhiza. 2011 Apr;21(3):145-54