Expression, purification, crystallization and preliminary X-ray analysis of the EIICGlc domain of the Escherichia coli glucose transporter.

Andreas Zurbriggen, Philipp Schneider, Priska Bähler, Ulrich Baumann, Bernhard Erni

Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland. andreas.zurbriggen@ibc.unibe.ch

Acta crystallographica. Section F, Structural biology and crystallization communications 2010 Jun 1

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The glucose-import system of Escherichia coli consists of a hydrophilic EIIA(Glc) subunit and a transmembrane EIICB(Glc) subunit. EIICB(Glc) (UniProt P69786) contains two domains: the transmembrane EIIC(Glc) domain (40.6 kDa) and the cytoplasmic EIIB(Glc) domain (8.0 kDa), which are fused by a linker that is strongly conserved among its orthologues. The EIICB(Glc) subunit can be split within this motif by trypsin. Here, the crystallization of the tryptic EIIC(Glc) domain is described. A complete data set was collected to 4.5 A resolution at 100 K.

Citation

Andreas Zurbriggen, Philipp Schneider, Priska Bähler, Ulrich Baumann, Bernhard Erni. Expression, purification, crystallization and preliminary X-ray analysis of the EIICGlc domain of the Escherichia coli glucose transporter. Acta crystallographica. Section F, Structural biology and crystallization communications. 2010 Jun 1;66(Pt 6):684-8