Generating retinal neurons by reprogramming retinal pigment epithelial cells.

Shu-Zhen Wang, Wenxin Ma, Run-Tao Yan, Weiming Mao

University of Alabama at Birmingham, Department of Ophthalmology, Birmingham, AL 35294-0009, USA. szwang@uab.edu

Expert opinion on biological therapy 2010 Aug

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Retinal degenerations cause blindness. One potential therapy is cell replacement. Because the human retina lacks regeneration capacity, much attention has been directed towards searching for cells that can differentiate into retinal neurons. We discuss the possibility of using transcription factor genes to channel retinal pigment epithelial (RPE) cells' capabilities of proliferation and plasticity towards the production of retinal neurons. Experiments with chick embryos show that RPE cells - in the eye, in explant, or in a dissociated cell culture - can give rise to cells resembling retinal neurons when reprogrammed with regulatory genes involved in retinal neurogenesis. Depending on the regulatory gene used, reprogramming generates cells exhibiting traits of photoreceptor cells, amacrine cells and/or young ganglion neurons. Gene-directed reprogramming of chick RPE can efficiently generate cells that exhibit traits of retinal neurons. Remaining to be addressed is the question of whether the results from chicks apply to mammals. Since the RPE is located adjacent to the neural retina, RPE reprogramming, if successful in mammals, may offer an approach to repopulate the neural retina without involving cell transplantation.

Citation

Shu-Zhen Wang, Wenxin Ma, Run-Tao Yan, Weiming Mao. Generating retinal neurons by reprogramming retinal pigment epithelial cells. Expert opinion on biological therapy. 2010 Aug;10(8):1227-39