Oxidative stress in response to forearm ischemia-reperfusion with and without carnitine administration.

Richard J Bloomer, Webb A Smith, Kelsey H Fisher-Wellman

Department of Health and Sport Sciences, The University of Memphis, Memphis TN 38152, USA. rbloomer@memphis.edu

International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition 2010 Jan

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We have recently noted a decrease in lipid peroxidation with oral intake of glycine propionyl-L-carnitine (GPLC). However, these findings were observed at rest, and in previously sedentary subjects. Methods: We determined the effect of GPLC on oxidative stress biomarkers at rest and in response to reactive hyperemia in exercise-trained men. Using a double-blind, crossover design, 15 healthy men were assigned to a placebo and GPLC (4.5 g/day) in random order, for four weeks, with a two-week washout between assignments. Blood samples were collected at rest and at 0, 3, and 10 minutes following a protocol of ischemia-reperfusion, and analyzed for lactate, malondialdehyde (MDA), F(2)-isoprostanes (F(2)-iso), hydrogen peroxide (H(2)O(2)), xanthine oxidase activity (XO), hypoxanthine (HYPO), total (TGSH) and oxidized (GSSG) glut athione, and Trolox-equivalent antioxidant capacity (TEAC). Results: No condition or condition by time interaction effects were noted (p>0.05). Time effects were noted for lactate (p<0.0001), MDA (p=0.02), H(2)O(2) (p=0.0003), XO (p=0.03), HYPO (p<0.0001), TGSH (p=0.02), and GSSG (p<0.0001), with peak values noted at 0 minutes post for lactate, MDA, TGSH, and GSSG, at 3 minutes post for H(2)O(2) and XO, and at 10 minutes post for HYPO. F(2)-iso and TEAC were unaffected by treatment or protocol (p>0.05). Conclusion: Short-term ischemia-reperfusion in trained men results in a modest and transient increase in selected blood oxidative stress biomarkers. Oral GPLC supplementation does not attenuate the increase in these biomarkers.

Citation

Richard J Bloomer, Webb A Smith, Kelsey H Fisher-Wellman. Oxidative stress in response to forearm ischemia-reperfusion with and without carnitine administration. International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition. 2010 Jan;80(1):12-23