Preparation and properties of SiO2 tubes immobilized antibody for HCAg detection].

In order to optimize the fabrication of SiO2 tubes immobilized with antibody for hepatitis C virus antigen (HCAg) detection, we formed the activated amino on the surface of SiO2 tubes by using the activation of aminosilane. Then we immobilized the hepatitis C virus (HCV) monoclonal antibody on the surface of SiO2 tubes by using glutaraldehyde as a chemical cross-linker, followed by detecting HCAg. Sequence tests showed that when the SiO2 tubes were treated in 10% (V/V) aminosilane solution and 3% (V/V) glutaraldehyde solution for 3 hours and 2 hours, respectively, the HCV monoclonal antibody had high immobilization efficiency and low nonspecificity, and the HCAg was detected to 1 ng/mL. This experiment can provide principle and experimental data for establishment of HCAg magnetic immunoassay system.

Citation

Li Xie, Yueping Guan, Ying Ge, Hongbo Shi. Preparation and properties of SiO2 tubes immobilized antibody for HCAg detection]. Sheng wu gong cheng xue bao = Chinese journal of biotechnology. 2010 Apr;26(4):545-9

Mesh Tags

Substances

PMID: 20575445

search → result