Effect of mitogen lectin on lymphocyte or brain cortex cell activation.

Hana Kovaru, Frantisek Kovaru, Marek Pav, Rudolf Dvorak, Miloslava Palikova, Jiri Raboch, Jarmila Zidková

1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. hkova@lf1.cuni.cz

Neuro endocrinology letters 2010

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We studied a) mitogen lectin (PHA) evoked changes of Na+/K+-ATPase activity in functionally different lymphocytes or brain cortex cells and b) quantitative relationship between PHA- evoked early enzyme activation and late lymphocyte proliferation were analyzed. We performed biochemical analyses of Pi released from ATP by Na+/K+-ATPase activity. Lymphocyte proliferation was assayed by 3H-thymidine incorporation. We demonstrated PHA stimulated Na+/K+-ATPase activity of mouse spleen lymphocytes or freshly isolated brain cortex cells. Besides this, we estimated high stimulation of Na+/K+-ATPase activity and subsequent late 3H-thymidine incorporation into pig lymphocytes as both PHA dose and K+ ion concentration dependent. Thus, early PHA dose-dependent stimulation of Na+/K+-ATPase activity is a more general response in different animal species and functionally different cells. We measured both cell type- and PHA-dose dependent enzyme activity stimulation. We can suggest that intensity of early PHA induced Na+/K+-ATPase activation could be in relationship to subsequent elevated level of T lymphocyte proliferation. The Na+/K+-ATPase can be a part of mitogen lectin evoked signal transduction mechanisms.

Citation

Hana Kovaru, Frantisek Kovaru, Marek Pav, Rudolf Dvorak, Miloslava Palikova, Jiri Raboch, Jarmila Zidková. Effect of mitogen lectin on lymphocyte or brain cortex cell activation. Neuro endocrinology letters. 2010;31(3):325-9