D-Tyr-tRNA(Tyr) deacylase, a new role in Alzheimer's-associated disease in SAMP8 mice.

Wei Liu, Chang Liu, Jing-xi Zhu, Ai-hua Li, Zhi-qiang Zhao, Bin Yin, Xiao-zhong Peng

National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China.

Chinese medical sciences journal = Chung-kuo i hsüeh k'o hsüeh tsa chih / Chinese Academy of Medical Sciences 2010 Jun

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To assess the expression level of D-Tyr-tRNA(Tyr) deacylase (DTD) in SAMP8 mice and speculate the function of DTD in disorders associated with Alzheimer's disease (AD). Altogether 12 SAMP8 mice and 12 SAMR1 mice were used in this study. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were performed to detect the mRNA and protein levels of DTD in the mice. Purified DTD protein was injected into lateral ventricle to investigate the function of DTD in SAMP mice. The behavior of the mice was tested by using a Step-through Test System. Both mRNA and protein levels of DTD were found to be significantly lower in SAMP8 mice compared with those in SAMR1 mice (P<0.05). In vivo injection of DTD protein did not lead to an obvious change in behavior of SAM mice. DTD might function in the process of AD-associated pathology and could possibly participate in physiology process in a long-term manner to orchestrate with other regulators in order to maintain the balance of organism.

Citation

Wei Liu, Chang Liu, Jing-xi Zhu, Ai-hua Li, Zhi-qiang Zhao, Bin Yin, Xiao-zhong Peng. D-Tyr-tRNA(Tyr) deacylase, a new role in Alzheimer's-associated disease in SAMP8 mice. Chinese medical sciences journal = Chung-kuo i hsüeh k'o hsüeh tsa chih / Chinese Academy of Medical Sciences. 2010 Jun;25(2):90-4