Complex phenotypes of a mutant inactivated for CymR, the global regulator of cysteine metabolism in Bacillus subtilis.

Marie-Françoise Hullo, Isabelle Martin-Verstraete, Olga Soutourina

Unité de Génétique des Génomes Bactériens, Institut Pasteur, Paris, France.

FEMS microbiology letters 2010 Aug 1

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We characterized various phenotypes of a mutant inactivated for CymR, the master regulator of cysteine metabolism in Bacillus subtilis. The deletion of cymR resulted in impaired growth in the presence of cystine and increased sensitivity to hydrogen peroxide-, disulfide-, paraquat- and tellurite-induced stresses. Estimation of metabolite pools suggested that these phenotypes could be the result of profound metabolic changes in the DeltacymR mutant including an increase of the intracellular cysteine pool and hydrogen sulfide formation, as well as a depletion of branched-chain amino acids.

Citation

Marie-Françoise Hullo, Isabelle Martin-Verstraete, Olga Soutourina. Complex phenotypes of a mutant inactivated for CymR, the global regulator of cysteine metabolism in Bacillus subtilis. FEMS microbiology letters. 2010 Aug 1;309(2):201-7