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We report a method using Trypan blue staining to detect primo-vessels in the nervous system on internal organs or in the skin of rat. We applied this technique to visualize the primo-vessels and primo-nodes in the brain, spinal cord and sciatic nerve of a rat. Primo-vessels and primo-nodes were preferentially stained at nerves, blood vessels, or fascia-like membranes and turned blue after the spread and washing of Trypan blue. The physiological role of the primo-vessels within the nervous system is an important question warranting further investigation. Copyright 2010 Korean Pharmacopuncture Institute. Published by .. All rights reserved.

Citation

Byung-Cheon Lee, Ki-Hoon Eom, Kwang-Sup Soh. Primo-vessels and primo-nodes in rat brain, spine and sciatic nerve. Journal of acupuncture and meridian studies. 2010 Jun;3(2):111-5

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PMID: 20633524

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