Peptide mimicry of AICL inhibits cytolysis of NK cells by blocking NKp80-AICL recognition.

Haihua Huang, Xiaodong Zheng, Zhigang Tian, Rui Sun

Institute of Immunology, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China.

Immunological investigations 2010

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AICL has been identified as a ligand of the activating NK cell receptor NKp80, but the interaction sites of NKp80-AICL were unknown. In this study, a 3-D model of AICL was constructed by using online server 3D-JIGSAW. Three highly conserved sequences of AICL on the surface of the AICL 3-D model were synthesized, and named as P1, P2 and P3, respectively. Flow cytometric analysis demonstrated that these peptides were able to compete with anti-NKp80 mAb on NKp80 binding activity in a dose-dependent manner. Moreover, P1 or P2 exerted inhibitory effects on NKp80-AICL mediated cytotoxicity of both fresh PBMCs and purified NK cells in (51)Cr release cytotoxicity assay. These results demonstrated that P1 and P2 sequences on AICL might be considered as the potential sites of NKp80-AICL interaction.

Citation

Haihua Huang, Xiaodong Zheng, Zhigang Tian, Rui Sun. Peptide mimicry of AICL inhibits cytolysis of NK cells by blocking NKp80-AICL recognition. Immunological investigations. 2010;39(6):587-97