A fungicidal piperazine-1-carboxamidine induces mitochondrial fission-dependent apoptosis in yeast.

Anna Bink, Gilmer Govaert, Isabelle E J A François, Klaartje Pellens, Lieven Meerpoel, Marcel Borgers, Geert Van Minnebruggen, Valérie Vroome, Bruno P A Cammue, Karin Thevissen

Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Heverlee, Belgium.

FEMS yeast research 2010 Nov

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To unravel the working mechanism of the fungicidal piperazine-1-carboxamidine derivative BAR0329, we found that its intracellular accumulation in Saccharomyces cerevisiae is dependent on functional lipid rafts. Moreover, BAR0329 induced caspase-dependent apoptosis in yeast, in which the mitochondrial fission machinery consisting of Fis1 (Whi2), Dnm1 and Mdv1 is involved. Our data are consistent with a prosurvival function of Fis1 (Whi2) and a proapoptotic function of Dnm1 and Mdv1 during BAR0329-induced yeast cell death.

Citation

Anna Bink, Gilmer Govaert, Isabelle E J A François, Klaartje Pellens, Lieven Meerpoel, Marcel Borgers, Geert Van Minnebruggen, Valérie Vroome, Bruno P A Cammue, Karin Thevissen. A fungicidal piperazine-1-carboxamidine induces mitochondrial fission-dependent apoptosis in yeast. FEMS yeast research. 2010 Nov;10(7):812-8