Department of Chemistry, Stanford University, Stanford, CA, USA.
Methods in molecular biology (Clifton, N.J.) 2011The normal function of neurons depends on the integrity of microtubule-dependent transport of cellular materials and organelles to/from their cell bodies or axon terminus. In this chapter, we describe the design and implementation of a fluorescence imaging method to visualize axonal transport in neurons directly. We combine a pseudo total internal reflection microscopy, quantum dot fluorescence labeling, microfluidic neuronal culture chamber, and single molecule detection methods to achieve a high spatial and temporal resolution in tracking nerve growth factor transport in dorsal root ganglia neurons.
Yasuko Osakada, Bianxiao Cui. Real-time visualization of axonal transport in neurons. Methods in molecular biology (Clifton, N.J.). 2011;670:231-43
PMID: 20967594
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