Rapid detection of Shigella dysenteriae by PCR assay].

Hongyuan Chen, Qingping Zhong, Li Wang, Yuanming Sun

The Higher Education Key Lab of Guangdong Province Food Quality and Safety, Institute of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.

Wei sheng yan jiu = Journal of hygiene research 2010 Sep

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Based on the invasive plasmid antigen H gene (ipaH) of S. dysenteriae, one pair of specific primers was designed for PCR assays in this study. The concentrations of dNTP, Mg2+ and primer, dosage of Taq DNA polymerase, annealing temperature and circulating parameter in the PCR amplification system were optimized. In this way, a rapid and stable method of PCR assay for the detection of S. dysenteriae was established. The specificity and sensitivity of PCR were also analyzed. The detection limits of pure culture and genomic DNA in the PCR assay were 1.06 x 10(2) cfu/ml and 106.34 pg/PCR system, respectively. The detection limit for S. dysenteriae in artificially contaminated food samples was 3.21 x 10(4) cfu/ml. These results indicated that the PCR method for S. dysenteriae detection was simple, rapid, high in specificity and sensitivity and suitable for the detection of pathogens in foods caused by Shigella dysenteriae.

Citation

Hongyuan Chen, Qingping Zhong, Li Wang, Yuanming Sun. Rapid detection of Shigella dysenteriae by PCR assay]. Wei sheng yan jiu = Journal of hygiene research. 2010 Sep;39(5):597-600