Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis.

Biaoming Zhang, Xupeng Cao, Xiaofei Cheng, Peichun Wu, Tonghu Xiao, Wei Zhang

Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.

Biotechnology letters 2011 Mar

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A novel, simple and highly efficient process for purifying vanadium bromoperoxidase from Corallina officinalis is reported. The key innovation is adding 0.5 mM sodium orthovanadate to the crude cell extract followed by heating at 70°C for 2 h, by which a 5.4-fold purification with a 100% activity recovery was achieved. Combining the heat treatment with ammonium sulfate precipitation and DEAE-52 column chromatography, the overall yield was 84%, 3.8 times greater than the highest yield previously reported. Finally, a specific activity of 310 U/mg, a 27-fold purification of the crude enzyme solution was produced.

Citation

Biaoming Zhang, Xupeng Cao, Xiaofei Cheng, Peichun Wu, Tonghu Xiao, Wei Zhang. Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis. Biotechnology letters. 2011 Mar;33(3):545-8