Alphacoronavirus transmissible gastroenteritis virus nsp1 protein suppresses protein translation in mammalian cells and in cell-free HeLa cell extracts but not in rabbit reticulocyte lysate.

Cheng Huang, Kumari G Lokugamage, Janet M Rozovics, Krishna Narayanan, Bert L Semler, Shinji Makino

Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, TX 77555-1019, USA.

Journal of virology 2011 Jan

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The nsp1 protein of transmissible gastroenteritis virus (TGEV), an alphacoronavirus, efficiently suppressed protein synthesis in mammalian cells. Unlike the nsp1 protein of severe acute respiratory syndrome coronavirus, a betacoronavirus, the TGEV nsp1 protein was unable to bind 40S ribosomal subunits or promote host mRNA degradation. TGEV nsp1 also suppressed protein translation in cell-free HeLa cell extract; however, it did not affect translation in rabbit reticulocyte lysate (RRL). Our data suggested that HeLa cell extracts and cultured host cells, but not RRL, contain a host factor(s) that is essential for TGEV nsp1-induced translational suppression.

Citation

Cheng Huang, Kumari G Lokugamage, Janet M Rozovics, Krishna Narayanan, Bert L Semler, Shinji Makino. Alphacoronavirus transmissible gastroenteritis virus nsp1 protein suppresses protein translation in mammalian cells and in cell-free HeLa cell extracts but not in rabbit reticulocyte lysate. Journal of virology. 2011 Jan;85(1):638-43