DNA binding and gel electrophoresis studies of a new silver(I) complex containing 2,9-dimethyl-1,10-phenanthroline ligands.

Nahid Shahabadi, Soheila Kashanian, Zeinab Ahmadipour

Faculty of Chemistry, Razi University, Kermanshah, Iran. nahidshahabadi@yahoo.com

DNA and cell biology 2011 Mar

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The silver(I) complex, [Ag(2,9-dimethyl-1,10-phenanthroline)(2)](NO(3)) · H(2)O, has been synthesized and characterized by physicochemical and spectroscopic methods. The binding interactions of this complex with calf thymus DNA (CT-DNA) were investigated using emission, absorption, circular dichroism, viscosity measurements, and gel electrophoresis studies. The calculated binding constant, K(b), obtained from UV-vis absorption studies was 5.3 ± 0.2 × 10(4) M(-1). In fluorimetric studies, the enthalpy and entropy of the reaction between the complex and CT-DNA showed hydrophobic interaction. In addition, in the circular dichroism spectrum, silver(I) complex induces a B → A structural transition of CT-DNA. Gel electrophoresis studies demonstrated that this complex has ability to cleave the supercoiled plasmid DNA</a>. All these results suggest that the complex interacts with CT-DNA via partial intercalative mode of binding.

Citation

Nahid Shahabadi, Soheila Kashanian, Zeinab Ahmadipour. DNA binding and gel electrophoresis studies of a new silver(I) complex containing 2,9-dimethyl-1,10-phenanthroline ligands. DNA and cell biology. 2011 Mar;30(3):187-94