Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3' → 5' exonuclease activity.

A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3' → 5' exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1.

Citation

Chung-Hang Leung, Daniel Shiu-Hin Chan, Bradley Yat-Wah Man, Chuan-Jen Wang, Wing Lam, Yung-Chi Cheng, Wang-Fun Fong, Wen-Luan Wendy Hsiao, Dik-Lung Ma. Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3' → 5' exonuclease activity. Analytical chemistry. 2011 Jan 15;83(2):463-6

Mesh Tags

Substances

PMID: 21114271

search → result