Extracellular production of a sphingomyelinase from Streptomyces griseocarneus using Streptomyces lividans.

Daisuke Sugimori, Yu Tomita, Yusaku Matsumoto, Chiaki Ogino

Department of Symbiotic Systems Science and Technology, Graduate School of Symbiotic Systems Science and Technology, Fukushima University, Kanayagawa, Fukushima, Japan. sugimori@sss.fukushima-u.ac.jp

Biotechnology letters 2011 Apr

filter terms:

The structural gene for sphingomyelinase (SMase) from Streptomyces griseocarneus, was introduced into Streptomyces lividans using a shuttle vector, pUC702, for Escherichia coli/S. lividans. High-level secretory production of SMase was achieved using the promoter, signal sequence and terminator regions of phospholipase D from Streptoverticillium cinnamoneum. The transformant constitutively expressed a high specific activity of SMase extracellularly during batch culture. Maximum SMase activity (555 ± 114 U/mg protein) was with 1.75 M MgCl(2) which was about 50-fold more than that with 10 mM MgCl(2).

Citation

Daisuke Sugimori, Yu Tomita, Yusaku Matsumoto, Chiaki Ogino. Extracellular production of a sphingomyelinase from Streptomyces griseocarneus using Streptomyces lividans. Biotechnology letters. 2011 Apr;33(4):727-31