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    We have purified flavin mononucleotide (FMN) from a flavoprotein-overexpressing Escherichia coli strain by cofactor trapping. This approach uses an overexpressed flavoprotein to trap FMN, which is thus removed from the cascade regulating FMN production in E. coli. This, in turn, allows the isolation of highly pure FMN.

    Citation

    Ulrich Krauss, Vera Svensson, Astrid Wirtz, Esther Knieps-Grünhagen, Karl-Erich Jaeger. Cofactor trapping, a new method to produce flavin mononucleotide. Applied and environmental microbiology. 2011 Feb;77(3):1097-100

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    PMID: 21131527

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