The effect of glycogen phosphorolysis on basal glutaminergic transmission.

Jerzy Mozrzymas, Tomasz Szczęsny, Darek Rakus

Department of Animal Molecular Physiology and Laboratory of Cellular Neurobiology, Institute of Zoology, Wrocław University, Cybulskiego 30, 50-205 Wrocław, Poland.

Biochemical and biophysical research communications 2011 Jan 14

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Astrocytic glycogen metabolism sustains neuronal activity but its impact on basal glutamatergic synaptic transmission is not clear. To address this issue, we have compared the effect of glycogen breakdown inhibition on miniature excitatory postsynaptic currents (mEPSCs) in rat hippocampal pure neuronal culture (PNC) and in astrocyte-neuronal co-cultures (ANCC). Amplitudes of mEPSC in ANCC were nearly twice as large as in PNC with no difference in current kinetics. Inhibition of glycogen phosphorylase reduced mEPSC amplitude by roughly 40% in ANCC being ineffective in PNC. Altogether, these data indicate that astrocyte-neuronal interaction enhances basal mEPSCs in ANCC mainly due to astrocytic glycogen metabolism. Copyright © 2010 Elsevier Inc. All rights reserved.

Citation

Jerzy Mozrzymas, Tomasz Szczęsny, Darek Rakus. The effect of glycogen phosphorolysis on basal glutaminergic transmission. Biochemical and biophysical research communications. 2011 Jan 14;404(2):652-5