Light regulation of protein dimerization and kinase activity in living cells using photocaged rapamycin and engineered FKBP.

Andrei V Karginov, Yan Zou, David Shirvanyants, Pradeep Kota, Nikolay V Dokholyan, Douglas D Young, Klaus M Hahn, Alexander Deiters

Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States.

Journal of the American Chemical Society 2011 Jan 26

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We developed a new system for light-induced protein dimerization in living cells using a photocaged analogue of rapamycin together with an engineered rapamycin binding domain. Using focal adhesion kinase as a target, we demonstrated successful light-mediated regulation of protein interaction and localization in living cells. Modification of this approach enabled light-triggered activation of a protein kinase and initiation of kinase-induced phenotypic changes in vivo.

Citation

Andrei V Karginov, Yan Zou, David Shirvanyants, Pradeep Kota, Nikolay V Dokholyan, Douglas D Young, Klaus M Hahn, Alexander Deiters. Light regulation of protein dimerization and kinase activity in living cells using photocaged rapamycin and engineered FKBP. Journal of the American Chemical Society. 2011 Jan 26;133(3):420-3