Epigenetic modification of retinoic acid-treated human embryonic stem cells.

Hyun Sub Cheong, Han Chul Lee, Byung Lae Park, Hyemin Kim, Mi Jin Jang, Yong Mahn Han, Seun-Young Kim, Yong Sung Kim, Hyoung Doo Shin

Department of Genetic Epidemiology, SNP Genetics, Inc., Seoul 153-803, Korea.

BMB reports 2010 Dec

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Epigenetic modification of the genome through DNA methylation is the key to maintaining the differentiated state of human embryonic stem cells (hESCs), and it must be reset during differentiation by retinoic acid (RA) treatment. A genome-wide methylation/gene expression assay was performed in order to identify epigenetic modifications of RA-treated hESCs. Between undifferentiated and RA-treated hESCs, 166 differentially methylated CpG sites and 2,013 differentially expressed genes were discovered. Combined analysis of methylation and expression data revealed that 19 genes (STAP2, VAMP8, C10orf26, WFIKKN1, ELF3, C1QTNF6, C10orf10, MRGPRF, ARSE, LSAMP, CENTD3, LDB2, POU5F1, GSPT2, THY1, ZNF574, MSX1, SCMH1, and RARB) were highly correlated with each other. The results provided in this study will facilitate future investigations into the interplay between DNA methylation and gene expression through further functional and biological studies.

Citation

Hyun Sub Cheong, Han Chul Lee, Byung Lae Park, Hyemin Kim, Mi Jin Jang, Yong Mahn Han, Seun-Young Kim, Yong Sung Kim, Hyoung Doo Shin. Epigenetic modification of retinoic acid-treated human embryonic stem cells. BMB reports. 2010 Dec;43(12):830-5