Cyclin-dependent kinase inhibitor p16 and p21 expression, and cell cycle change in human lens epithelial cell line SRA 01/04 following contact inhibition in normal culture.

Yoko Yamashiro, Hiroshi Sasaki, Nobuhiro Ibaraki, Kota Nagai, Yutaka Kawakami, Hiromoto Yaguchi, Nobuyuki Fujita, Hiromi Osada, Kazuyuki Sasaki

Division of Vision Research for Environmental Health, Medical Research Institute, Kanazawa Medical University, Uchinada, Japan. yamashir@kanazawa-med.ac.jp

Ophthalmic research 2011

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To describe the pattern of expression of the cyclin-dependent kinase inhibitors (CDKIs) p16, p21 and p27, and the cell cycle in SRA 01/04 cells relative to contact inhibition. SRA 01/04 cells were grown to overconfluence under normal conditions. At various phases of the cell growth, cells were assayed by flow cytometry and Western blotting for the expression of CDKIs. Expression of p16 was detected from early logarithmic growth to stationary phases, during which the number of cells in G(0)/G(1) increased from 46 to 69%. Expression of p21 was detected only during the overgrowth phase, when 60% of the cells were in G(0)/G(1). Expression of p27 was not observed in SRA 01/04 cells. p16 expression was likely mediated by G(0)/G(1) arrest to induce contact inhibition in SRA 01/04. p21 expression may be related to withdrawal, and p27 deficiency may be related to the immortality of this cell line. It is possible for p16 to stop proliferation of lens epithelial cells like progressing posterior capsular opacification, by overexpression to mimic contact inhibition. Copyright © 2011 S. Karger AG, Basel.

Citation

Yoko Yamashiro, Hiroshi Sasaki, Nobuhiro Ibaraki, Kota Nagai, Yutaka Kawakami, Hiromoto Yaguchi, Nobuyuki Fujita, Hiromi Osada, Kazuyuki Sasaki. Cyclin-dependent kinase inhibitor p16 and p21 expression, and cell cycle change in human lens epithelial cell line SRA 01/04 following contact inhibition in normal culture. Ophthalmic research. 2011;46(1):38-43