Structural characterization of the multidomain regulatory protein Rv1364c from Mycobacterium tuberculosis.

The open reading frame rv1364c of Mycobacterium tuberculosis, which regulates the stress-dependent σ factor, σ(F), has been analyzed structurally and functionally. Rv1364c contains domains with sequence similarity to the RsbP/RsbW/RsbV regulatory system of the stress-response σ factor of Bacillus subtilis. Rv1364c contains, sequentially, a PAS domain (which shows sequence similarity to the PAS domain of the B. subtilis RsbP protein), an active phosphatase domain, a kinase (anti-σ(F) like) domain and a C-terminal anti-σ(F) antagonist like domain. The crystal structures of two PAS domain constructs (at 2.3 and 1.6 Å) and a phosphatase/kinase dual domain construct (at 2.6 Å) are described. The PAS domain is shown to bind palmitic acid but to have 100 times greater affinity for palmitoleic acid. The full-length protein can exist in solution as both monomer and dimer. We speculate that a switch between monomer and dimer, possibly resulting from fatty acid binding, affects the accessibility of the serine of the C-terminal, anti-σ(F) antagonist domain for dephosphorylation by the phosphatase domain thus indirectly altering the availability of σ(F). Copyright © 2011 Elsevier Ltd. All rights reserved.

Citation

Jack King-Scott, Petr V Konarev, Santosh Panjikar, Rositsa Jordanova, Dmitri I Svergun, Paul A Tucker. Structural characterization of the multidomain regulatory protein Rv1364c from Mycobacterium tuberculosis. Structure (London, England : 1993). 2011 Jan 12;19(1):56-69

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PMID: 21220116

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