Carbohydrate gel electrophoresis.

Florence Goubet, Paul Dupree, Katja Salomon Johansen

Bayer BioScience NV, Ghent, Belgium. Florence.Goubet@bayercropscience.com

Methods in molecular biology (Clifton, N.J.) 2011

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Polysaccharide analysis using carbohydrate gel electrophoresis (PACE) relies on derivatization of the reducing ends of sugars with a fluorophore, followed by electrophoresis under optimized conditions in polyacrylamide gels. PACE is a sensitive and simple tool for studying polysaccharide structure or quantity and also has applications in the investigation of enzyme specificity.

Citation

Florence Goubet, Paul Dupree, Katja Salomon Johansen. Carbohydrate gel electrophoresis. Methods in molecular biology (Clifton, N.J.). 2011;715:81-92