Synthesis and evaluation of anti-tubercular activity of new dithiocarbamate sugar derivatives.

Yasuhiro Horita, Takemasa Takii, Ryuji Kuroishi, Taku Chiba, Kenji Ogawa, Laurent Kremer, Yasuo Sato, YooSa Lee, Tomohiro Hasegawa, Kikuo Onozaki

Department of Molecular Health Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.

Bioorganic & medicinal chemistry letters 2011 Feb 1

filter terms:

The present study was undertaken to optimize the anti-tubercular activity of 2-acetamido-2-deoxy-β-D-glucopyranosyl N,N-dimethyldithiocarbamate (OCT313, Glc-NAc-DMDC), a lead compound previously reported by us. Structural modifications of OCT313 included the replacements of the DMDC group at C-1 by pyrrolidine dithiocarbamate (PDTC) and the acetyl group at C-2 by either propyl, butyl, benzyl or oleic acid groups. The antimycobacterial activities of these derivatives were evaluated against Mycobacterium tuberculosis (MTB). Glc-NAc-pyrrolidine dithiocarbamate (OCT313HK, Glc-NAc-PDTC) exhibited the most potent anti-tubercular activity with the minimal inhibitory concentration (MIC) of 6.25-12.5 μg/ml. The antibacterial activity of OCT313HK was highly specific to MTB and Mycobacterium bovis BCG, but not against Mycobacterium avium, Mycobacterium smegmatis, Staphylococcus aureus or Escherichia coli. Importantly, OCT313HK was also effective against MTB clinical isolates, including multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains. Interestingly, OCT313HK was exerted the primary bactericidal activity, and it was also exhibited the bacteriolytic activity at high concentrations. We next investigated whether the mycobacterial monooxygenase EthA, a common activator of thiocarbamide-containing anti-tubercular drugs, also activated OCT313HK. Contrary to our expectations, the anti-tubercular activity of dithiocarbamate sugar derivatives and dithiocarbamates were not dependent on ethA expression, in contrast to thiocarbamide-containing drugs. Overall, this study presents OCT313HK as a novel and potent compound against MTB, particularly promising to overcome drug resistance. Copyright © 2010 Elsevier Ltd. All rights reserved.

Citation

Yasuhiro Horita, Takemasa Takii, Ryuji Kuroishi, Taku Chiba, Kenji Ogawa, Laurent Kremer, Yasuo Sato, YooSa Lee, Tomohiro Hasegawa, Kikuo Onozaki. Synthesis and evaluation of anti-tubercular activity of new dithiocarbamate sugar derivatives. Bioorganic & medicinal chemistry letters. 2011 Feb 1;21(3):899-903