Yan Fu, Xian Wang, Jinli Zhang, Ying Xiao, Wei Li, Jingkang Wang
Key Laboratory for Green Chemical Technology MOE, Tianjin University, Tianjin 300072, People's Republic of China.
Biomacromolecules 2011 Mar 14Spermine-induced orderly assembling properties of G-/C-rich oligonucleotides are investigated in dilute and crowding conditions. The first time we report that the parallel G-quadruplexes is preferential to condense into anisotropic microaggregates in the presence of spermine, whereas the hybrid-type and the antiparallel G-quadruplexes have no significant interactions with spermine; and spermine can induce the condensation of i-motif C-rich oligonucleotides other than the random coiled C-rich strands. Moreover, the condensation of C-rich oligonucleotides can be reversibly regulated by pH and temperature. G-/C-rich oligonucleotides exhibit the cholesteric liquid crystalline phase at low strand concentration in the presence of spermine under crowding conditions. The results illuminate that the parallel G-quadruplex and i-motifs are probably necessity conformations for G-/C-rich oligonucleotides that involved in the regulation of chromosome organization in living cells.
Yan Fu, Xian Wang, Jinli Zhang, Ying Xiao, Wei Li, Jingkang Wang. Orderly microaggregates of G-/C-rich oligonucleotides associated with spermine. Biomacromolecules. 2011 Mar 14;12(3):747-56
PMID: 21235226
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