The rate-limiting step of sulfiredoxin is associated with the transfer of the γ-phosphate of ATP to the sulfinic acid of overoxidized typical 2-Cys peroxiredoxins.

Xavier Roussel, Samia Boukhenouna, Sophie Rahuel-Clermont, Guy Branlant

Unité Mixte de Recherche CNRS-Université Henri Poincaré 7214 AREMS, Nancy Université, Faculté des Sciences et Technologies, Vandœuvre-lès-Nancy, France.

FEBS letters 2011 Feb 4

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The eukaryotic sulfiredoxin (Srx) catalyzes the reduction of overoxidized typical 2-Cys peroxiredoxins PrxSO(2) via ATP/Mg(2+)-dependent phosphorylation of the sulfinic acid group, followed by formation of a PrxSO-SSrx thiolsulfinate intermediate. Using real-time kinetics of wild-type and C84A Srxs, and pH-rate profiles with ATP/Mg(2+) analogues, we show that the rate-limiting step of the reaction is associated with the chemical process of transfer of the γ-phosphate of ATP to the sulfinic acid, in contrast to that described by Jönsson et al. Two pK(apps) of 6.2 and 7.5 were extracted from the bell-shaped pH-rate profile, corresponding to the γ-phosphate of ATP, and to an acid-base catalyst, respectively. Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Citation

Xavier Roussel, Samia Boukhenouna, Sophie Rahuel-Clermont, Guy Branlant. The rate-limiting step of sulfiredoxin is associated with the transfer of the γ-phosphate of ATP to the sulfinic acid of overoxidized typical 2-Cys peroxiredoxins. FEBS letters. 2011 Feb 4;585(3):574-8