Effect of perfluorohexane on the expression of cellular adhesion molecules and surfactant protein A in human mesothelial cells in vitro.

Dirk Haufe, Klaus G Dahmen, Oliver Tiebel, Matthias Hübler, Thea Koch

Department of Anesthesiology and Intensive Care Medicine, University Hospital Carl Gustav Carus, Technical University Dresden, Dresden, Germany. Dirk.Haufe@uniklinikum-dresden.de

Artificial cells, blood substitutes, and immobilization biotechnology 2011 Aug

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The intraperitoneal instillation of perfluorocarbons augmented systemic oxygenation and was protective in mesenteric ischemia-reperfusion and experimental lung injury. To study biocompatibility and potential anti-inflammatory effects of intraperitoneal perfluorocarbons, we evaluated the influence of perfluorohexane and/or inflammatory stimuli on human mesothelial cells in vitro. Perfluorohexane exposure neither impaired cell viability nor induced cellular activation. TNFα enhanced ICAM-1 expression, which was not attenuated by simultaneous perfluorohexane treatment. Concentration of intracellular surf actant protein A tended to be higher in perfluorohexane treated cells compared to controls. Our in vitro data add further evidence that intraperitoneal perfluorocarbon application is feasible without adverse local effects.

Citation

Dirk Haufe, Klaus G Dahmen, Oliver Tiebel, Matthias Hübler, Thea Koch. Effect of perfluorohexane on the expression of cellular adhesion molecules and surfactant protein A in human mesothelial cells in vitro. Artificial cells, blood substitutes, and immobilization biotechnology. 2011 Aug;39(4):239-46