Functional analysis of human sodium-phosphate transporter 4 (NPT4/SLC17A3) polymorphisms.

Promsuk Jutabha, Naohiko Anzai, Toru Kimura, Atsuo Taniguchi, Wako Urano, Hisashi Yamanaka, Hitoshi Endou, Hiroyuki Sakurai

Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Tokyo 181-8611, Japan.

Journal of pharmacological sciences 2011

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We analyzed the functional properties of five nonsynonymous single nucleotide polymorphisms (SNPs) in the sodium-phosphate transporter NPT4 gene (SLC17A3) using the Xenopus oocyte expression system. NPT4 variants carrying SNP V257F, G279R, or P378L exhibited reduced transport of [(14)C]para-aminohippurate, [(3)H]bumetanide, [(3)H]estrone sulfate, and [(14)C]urate, when each variant clone was expressed in the plasma membrane of oocytes. This study suggests the possibility that the genetic variation of NPT4 contributes to inter-individual differences in disposition of anionic drugs such as diuretics as well as certain endogenous organic anions such as urate.

Citation

Promsuk Jutabha, Naohiko Anzai, Toru Kimura, Atsuo Taniguchi, Wako Urano, Hisashi Yamanaka, Hitoshi Endou, Hiroyuki Sakurai. Functional analysis of human sodium-phosphate transporter 4 (NPT4/SLC17A3) polymorphisms. Journal of pharmacological sciences. 2011;115(2):249-53