Karin S Kassahn, Nic Waddell, Sean M Grimmond
Queensland Centre for Medical Genomics, Institute for Molecular Bioscience, Brisbane, Australia.
Integrative biology : quantitative biosciences from nano to macro 2011 MayThe development of next-generation sequencing technologies has enabled the transcriptome to be measured and characterized at a level which was previously unattainable. Shot gun sequencing of RNAs, or RNA-Seq as it is known, is providing the means to simultaneously survey locus activity, transcript-specific expression, sequence content of transcripts and transcriptome discovery. This article discusses the current state of RNA-Seq, its potential for redefining transcriptomics and some of the challenges associated with this revolutionary technology.
Karin S Kassahn, Nic Waddell, Sean M Grimmond. Sequencing transcriptomes in toto. Integrative biology : quantitative biosciences from nano to macro. 2011 May;3(5):522-8
PMID: 21298135
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