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The development of next-generation sequencing technologies has enabled the transcriptome to be measured and characterized at a level which was previously unattainable. Shot gun sequencing of RNAs, or RNA-Seq as it is known, is providing the means to simultaneously survey locus activity, transcript-specific expression, sequence content of transcripts and transcriptome discovery. This article discusses the current state of RNA-Seq, its potential for redefining transcriptomics and some of the challenges associated with this revolutionary technology.


Karin S Kassahn, Nic Waddell, Sean M Grimmond. Sequencing transcriptomes in toto. Integrative biology : quantitative biosciences from nano to macro. 2011 May;3(5):522-8

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PMID: 21298135

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