Suicide inactivation of tyrosinase in its action on tetrahydropterines.

Jose Luis Muñoz-Muñoz, Francisco Garcia-Molina, Enrique Arribas, Pedro Antonio Garcia-Ruíz, Jose Tudela, Francisco Garcia-Cánovas, Jose Neptuno Rodríguez-López

Grupo de Investigación Enzimología, Departamento de Bioquímica y Biología Molecular-A, Facultad de Biología, Universidad de Murcia, E-30100, Espinardo, Murcia, Spain.

Journal of enzyme inhibition and medicinal chemistry 2011 Oct

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Tetrahydrobiopterin (BH(4)), methyl-tetrahydropterin (MBH(4)) and dimethyl-tetrahydropterin (DMBH(4)) are oxidized by tyrosinase in a process during which the suicide inactivation of tyrosinase may occur. From the kinetic study of this process, [Formula: see text] (apparent maximum constant for the suicide inactivation), [Formula: see text] (Michaelis constant for the substrate) and r (number of turnovers that the enzyme makes before the inactivation) can be obtained. From the results obtained, it can be deduced that the velocity of the inactivation governed by ([Formula: see text]) and the potency of the same ([Formula: see text]) follow the order: BH(4) > MBH(4) > DMBH(4).

Citation

Jose Luis Muñoz-Muñoz, Francisco Garcia-Molina, Enrique Arribas, Pedro Antonio Garcia-Ruíz, Jose Tudela, Francisco Garcia-Cánovas, Jose Neptuno Rodríguez-López. Suicide inactivation of tyrosinase in its action on tetrahydropterines. Journal of enzyme inhibition and medicinal chemistry. 2011 Oct;26(5):728-33