Beta-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium].

Lilian Chuaire-Noack, Sandra Rondón-Lagos, Magda Sánchez-Corredor, Milcíades Ibáñez-Pinilla, Sandra Ramírez-Clavijo

Facultad de Ciencias Naturales y Matemáticas, Universidad del Rosario, Bogotá DC, Colombia. lilian.chuaire@urosario.edu.co

Investigación clínica 2010 Sep

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Beta-galactosidase activity reflects the rate of cellular aging in vitro. Such activity was quantified at pH 6 in ovarian epithelial cells from 28 donors without a history of cancer, by the chemoluminiscent method. The cells were serially cultured until they achieved the state of permanent growth arrest. During the exponential growth phase, all cultures showed a similar pattern of growth and low beta-galactosidase activity. However, both in the onset of decrease replicative activity, as well as in the onset of the stationary phase, there was a significant rise in the enzyme activity. Our results showed that beta-galactosidase activity can be considered as a replicative senescence marker of the ovarian surface epithelium at pH 6.

Citation

Lilian Chuaire-Noack, Sandra Rondón-Lagos, Magda Sánchez-Corredor, Milcíades Ibáñez-Pinilla, Sandra Ramírez-Clavijo. Beta-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium]. Investigación clínica. 2010 Sep;51(3):351-67