NDUFB7 and NDUFA8 are located at the intermembrane surface of complex I.

Radek Szklarczyk, Bas F J Wanschers, Sander B Nabuurs, Jessica Nouws, Leo G Nijtmans, Martijn A Huynen

Centre for Molecular and Biomolecular Informatics, Radboud University Nijmegen Medical Centre, 6500 HB, Nijmegen, The Netherlands.

FEBS letters 2011 Mar 9

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Complex I (NADH:ubiquinone oxidoreductase) is the first and largest protein complex of the oxidative phosphorylation. Crystal structures have elucidated the positions of most subunits of bacterial evolutionary origin in the complex, but the positions of the eukaryotic subunits are unknown. Based on the analysis of sequence conservation we propose intra-molecular disulfide bridges and the inter-membrane space localization of three Cx(9)C-containing subunits in human: NDUFS5, NDUFB7 and NDUFA8. We experimentally confirm the localization of the latter two, while our data are consistent with disulfide bridges in NDUFA8. We propose these subunits stabilize the membrane domain of complex I. Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Citation

Radek Szklarczyk, Bas F J Wanschers, Sander B Nabuurs, Jessica Nouws, Leo G Nijtmans, Martijn A Huynen. NDUFB7 and NDUFA8 are located at the intermembrane surface of complex I. FEBS letters. 2011 Mar 9;585(5):737-43