Switching of the enzymatic activity synchronized with signal recognition by an artificial DNA receptor on a liposomal membrane.

Yoshihiro Sasaki, Masaru Mukai, Akihiro Kawasaki, Kazuma Yasuhara, Jun-ichi Kikuchi

Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan. ysasaki.org@tmd.ac.jp

Organic & biomolecular chemistry 2011 Apr 7

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We constructed a supramolecular system on a liposomal membrane that is capable of activating an enzyme via DNA hybridization. The design of the system was inspired by natural signal transduction systems, in which enzymes amplify external signals to control signal transduction pathways. The liposomal membrane, providing a platform for the system, was prepared by the self-assembly of an oligonucleotide lipid, a phospholipid and a cationic synthetic lipid. The enzyme was immobilized on the liposomal surface through electrostatic interactions. Selective recognition of DNA signals was achieved by hybridizing the DNA signals with the oligonucleotide lipid embedded in the liposome. The hybridized DNA signal was sent to the enzyme by a copper ion acting as a mediator species. The enzyme then amplified the event by the catalytic reaction to generate the output signal. In addition, our system demonstrated potential for the discrimination of single nucleotide polymorphisms.

Citation

Yoshihiro Sasaki, Masaru Mukai, Akihiro Kawasaki, Kazuma Yasuhara, Jun-ichi Kikuchi. Switching of the enzymatic activity synchronized with signal recognition by an artificial DNA receptor on a liposomal membrane. Organic & biomolecular chemistry. 2011 Apr 7;9(7):2397-402