Moonlighting role of a poly-gamma-glutamate synthetase component from Bacillus subtilis: insight into novel extrachromosomal DNA maintenance.

Daisuke Yamashiro, Yutaka Minouchi, Makoto Ashiuchi

Course of Applied Bioresource Science, United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Ehime 790-8566, Japan.

Applied and environmental microbiology 2011 Apr

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The Bacillus subtilis structural gene pgsE was investigated as a tool for extrachromosomal DNA maintenance (EDM). It ameliorated the stability of high-copy-number vectors, regardless of whether they were derived from rolling-cycle or theta-mode replicons, without any selective pressure. This unique EDM phenomenon may occur via a trans-acting mechanism.

Citation

Daisuke Yamashiro, Yutaka Minouchi, Makoto Ashiuchi. Moonlighting role of a poly-gamma-glutamate synthetase component from Bacillus subtilis: insight into novel extrachromosomal DNA maintenance. Applied and environmental microbiology. 2011 Apr;77(8):2796-8